Fig. 2
From: Integrative single-cell RNA-seq analysis of vascularized cerebral organoids
Mapping of scRNA-seq data from non-vascularized and vascularized organoids and fetal brain to UMAP with cell types. A UMAP visualization of transcriptomes with cell types. Left panel: UMAP integrated scRNA-seq data for all samples, right panel: UMAP for each sample. Assigned cell types are as follows: PROG; Progenitors (SOX2+), PC; Proliferating Cells (TOP2A+, BIRC5+), IPC; Intermediate Progenitor Cells (EOMES+, PPP1R17+), Neuron (RBFOX3+, TUBB3+), IN; Inhibitory Neurons (GAD1+, GAD2+), EX; Excitatory Neurons (SLC17A6+, SLC17A7+), AS; Astrocytes (GFAP+, AQP4+), OPC; Oligodendrocyte Progenitor Cells (OLIG1+, OLIG2+), VLC; Vascular-Related-Like Cells (CLDN5+, COL1A1+, RGS5+, ACTA2+), MGLC; Microglia-like Cells (CD53+, CX3CR1+), and UPRC; Unfolded Protein Response Cells (DDIT3+). B Expression of marker genes of each cell type in each sample. The cell type is displayed on the x-axis, and the marker gene for each cell type is displayed on the y-axis. The color of the marker corresponds to the color of each cell type. hCO; human cerebral organoids, and vhCO; vascularized hCO. C Ratio of cells present in each sample. The ratio is calculated as the percentage of each cell count to the total cells in each sample