Fig. 4

(Sub-)Cellular expression of SHANK2 in the human and mouse brain. a–c Staining of SHANK2 is opposed for human (left) and mouse (right) tissue sections. Co-staining of cellular (a,b) and synaptic (c) proteins in the CB, SN, and the SL of the HC. Arrows in c highlight SHANK2-/VGLUT1 + synapses. Scale bars, 10 µm (a), 20 µm (b, left), 5 µm (b, right), 1 µm (c). d, e Additional co-staining in the human LC (d) and CB (e). Scale bars, 15 µm (d), 20 µm (e), 1 µm (insets e). ml = molecular layer, Pl = Purkinje cell layer, gl = granular layer. DAPI (blue) is overlaid in a, b and d, e. f Confocal and STED microscopy of the same chemical synapse. Individual channels are shown as smaller images on the right, the graph shows the intensity profile across a SHANK2-positive PSD (white line) in confocal (black curve) and STED mode (red curve). Intensities were normalized to the maximum. Scale bars, 0.5 µm. a.u. = arbitrary unit