Fig. 3

GADD45A affects adipogenesis in white preadipocytes. A ORO and BODIPY staining of total lipids in undifferentiated and differentiated mice subcutaneous preadipocytes. TG was measured (n = 3). B, C The expression of adipogenic genes and GADD45A was assessed by qPCR (B) (n = 3) and Western blotting (C). D Mice subcutaneous adipocytes were infected with control adenovirus (Ctrl) and adenovirus-expressing GADD45A (G45a-oe) and differentiated for 6 days. ORO and BODIPY staining of control and G45a-oe cells after induction of differentiation. Nuclei were stained with DAPI. TG was measured (n = 3). E, F qPCR and western blotting for the expression of GADD45A and adipogenic-related genes in control and G45a-oe cells (n = 3). G ORO and BODIPY staining in differentiated preadipocytes infected with mouse control shRNA (shCtrl) and GADD45A shRNA (shG45a). Nuclei were stained with DAPI (blue). OD490 was measured (n = 6). H The mRNA levels of related genes with GADD45A KD after differentiation (n = 4). Error bars represent SEM, *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed Student’s t-test. Scale bars: 100 μm and 50 μm