Fig. 6
From: GADD45A regulates subcutaneous fat deposition and lipid metabolism by interacting with Stat1
GADD45A prevented the phosphorylation of Stat1. A Immunofluorescence staining of phosphorylation of Stat1 in HEK293T cells. Cells were transfected with pCDNA3.1 or GADD45A plasmid. 24 h later, cells were treated for 30 min with hIFN-α1. Then, cells were stained with primary anti-pStat1 (Tyr701) and secondary species-specific FITC-conjugated antibodies, followed by Actin-Tracker and DAPI. B Western blotting analysis of whole cell lysate (WCL) and nuclear fractions in HEK293T cells with hIFN-α1 treatment. C Relative gray values for the phosphorylation of Stat1 (Tyr701) in B (n = 3). Error bars represent SEM, *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed Student’s t-test. Scale bars: 100 μm