Skip to main content
Fig. 1 | BMC Biology

Fig. 1

From: Removal of a partial genomic duplication restores synaptic transmission and behavior in the MyosinVA mutant mouse Flailer

Fig. 1

Targeting the Flailer genomic locus with DN-CRISPRs. A Scheme of gnb5, flailer, and myo5a genes. B Schematic representation of flailer editing. Black arrows indicate the region of the DNA targeted by sgRNAs to edit the flailer gene via DN-CRISPRs. sgRNAs for DN-CRISPRs need to be targeted at opposite strands. Red arrows show the primers used for PCR analysis to determine the status of the genomic locus. C PCR products to analyze gene editing of flailer-infected neurons. Protein (D) and mRNA (E) expression levels infected with FL1-FL5. F Immunofluorescences against PSD95 and Synaptophysin in wild-type, FL, and FL1-injected neurons. Bars represent mean ± SEM; ***p < 0.001. One-way ANOVA was used to determine significance

Back to article page

BMC Biology

ISSN: 1741-7007

Contact us