Fig. 2

Maximum-likelihood phylogenetic tree detailing the relationship between Puccinia striiformis f. sp. tritici isolates, annotated with their mating type alleles. Dendrogram is derived from 206,376 SNPs across all datasets not excluded for poor coverage (N=370/386). Mating type alleles present in the isolate are shown as a pair of coloured shapes in the ring surrounding the tree. Unknown mating types are represented with a black bar. Clades are derived from bootstrap and STRUCTURE analysis and are displayed as coloured branches, matching outer ring colours, and matching text labels. Clade labels are supplemented below with the mating types identified within the clade. Samples from southern Africa exhibit a single gene with the Pst-b1-HD mating type and another with the Pst-b1*-HD mating type and so are shown with split colours. Bootstrap support >80% is shown with a small blue dot at the node. gDNA samples are highlighted with a pink dot just after the branch tip and can be observed to form a tight cluster within their clade. The tree was generated using RAxML and visualized/annotated in IToL and Adobe Illustrator