Fig. 10

Venn diagram illustrating the identification of proteins in different eggshell proteomic data sets. Venn diagram comparing the number of identified eggshell proteins between the current study and previously published data by Marinotti et al. [6]. About 40% of the eggshell proteins from the previous study were found in the current study, while 168 additional proteins were detected from our eggshell proteomic analysis. Detailed identified proteins are listed in Additional file 11: Table S10. Eight putative protein functions were evaluated by comparing identified eggshell proteins from this study with previously published proteomics data by Marinotti et al. [6]. Proteins with more than or equal to six peptide hits from four independent proteomic data sets were included. Two independent biological replicates from both RNAi-Fluc and RNAi-EOF1 were used in the proteomic analysis. Eggshell peptide abundance fold changes in response to RNAi-EOF1 (AAEL012336) are shown in comparison with RNAi-Fluc control