Fig. 9

Scanning electron micrograph images of eggshell ultrastructural features from RNAi Aedes aegypti mosquitoes. A Dorsal view of an entire primary follicle from an RNAi-Fluc mosquito. The image was taken under × 600 magnification (scale bars = 50 μm). B Representative dorsal images are shown from RNAi-Fluc control (B), -Nudel (C), -Nasrat (D), -Closca (E), -Polehole (F), DCE2 (G), -DEC4 (H), -DCE5 (I), -CATL3 (J), and -EOF1 (K) mosquitoes. The photos were taken under × 6000 magnification (scale bars = 10 μm). CT, central tubercle; PT, peripheral tubercle; EN, exochorionic network; OCA, outer chorionic area. Mosquitoes were injected with dsRNA as in Fig. 1. Mature primary follicles were dissected in 1 × PBS at 96 h PBM, immediately fixed with 2.5% electron microscopy grade glutaraldehyde at 4 °C, post-fixed with 1% osmium tetroxide at room temperature, dehydrated with 100% ethanol, dried with hexamethyldisilazane, and metallized with gold. All dorsal images of eggs were taken using a FEI Inspect-S electron scanning microscope at the W.M. Keck Center for Nano-Scale Imaging, University of Arizona (RRID:SCR_022884)