Fig. 1

WD-induced immobility and anxiety-related behaviors in WT mice not exhibited in WD-fed Tas1r3^−/− mice. A Tas1r3 mRNA expression in the hypothalamus of all groups. Two-way ANOVA followed by Tukey’s multiple comparison test: *p < 0.05, ***p < 0.001. n = 3 mice/group. B Schematic diagram of the OF test. The inner blue dotted line denotes the center zone. C-E Locomotor activities assessed by total distance traveled in the arena, average speed and time spent immobile. Two-way ANOVA followed by Tukey’s multiple comparison test: *p < 0.05, **p < 0.01. F Representative movement traces (blue lines) in OF chamber. G,H Anxiety-related behaviors measured by the frequency of entries to the center zone, and distance traveled in the center zone. Two-way ANOVA followed by Tukey’s multiple comparison test: *p < 0.05, **p < 0.01. WT ND: n = 18; WT WD: n = 24; Tas1r3^−/− ND: n = 14; Tas1r3^−/− WD: n = 21. All values are presented as the means ± SEM. ANOVA, analysis of variance; OF, open field; ND, normal diet; WD, western diet; WT, wild-type