Fig. 2

Tas1r3 deficiency protects against the anxiety-like phenotypes induced by WD. A Schematic of EPM test. B Representative heatmaps of time spent in EPM. C Distance traveled in the open arms. Two-way ANOVA followed by Tukey’s multiple comparison test: **p < 0.01. D Time spent in open arms. Two-way ANOVA followed by Tukey’s multiple comparison test: *p < 0.05, **p < 0.01. E Time spent in closed arms. Two-way ANOVA followed by Tukey’s multiple comparison test: **p < 0.01, ***p < 0.001. WT ND: n = 18; WT WD: n = 24; Tas1r3^−/− ND: n = 14; Tas1r3^−/− WD: n = 21. F Schematic of LDB test. G Representative movement traces (blue lines) in LDB. H Time spent in light zone. Two-way ANOVA followed by Tukey’s multiple comparison test: *p < 0.05. WT ND: n = 9; WT WD: n = 11; Tas1r3^−/− ND: n = 9; Tas1r3^−/− WD: n = 11. I Schematic of NSF test. J Latency time to feed. Two-way ANOVA followed by Tukey’s multiple comparison test: *p < 0.05. WT ND: n = 9; WT WD: n = 11; Tas1r3^−/− ND: n = 9; Tas1r3^−/− WD: n = 11. All values are presented as the means ± SEM. ANOVA, analysis of variance; EPM, elevated plus maze; LDB, Light–dark box; ND, normal diet; NSF, novelty-suppressed feeding; WD, western diet; WT, wild-type