Fig. 4

WD-fed Tas1r3^−/− mice displayed maintained hypothalamic neurogenesis compared to WD-fed WT mice. A Significantly detected disease and function in WD-fed Tas1r3^−/− mice compared to WD-fed WT mice. Right-tailed Fisher’s exact test in IPA. Dots represent –log(p-value) and bars represent Z-score for each disease and function annotated. B GSEA related to neurogenesis. C qPCR validation of mRNA expression of neuronal progenitor cell markers and hypothalamus-related neuronal cell markers of the hypothalamus. Two-way ANOVA followed by Tukey’s multiple comparison test: *p < 0.05, **p < 0.01, and ***p < 0.001. n = 3 mice/group. D qPCR validation of Bax to Bcl-2 mRNA expression ratio. Two-way ANOVA followed by Tukey’s multiple comparison test: **p < 0.01, ***p < 0.001. n = 3 mice/group. E DCX staining of regions of the hypothalamus in each group. F The number of DCX-positive cells per field in regions of the hypothalamus. Two-way ANOVA followed by Tukey’s multiple comparison test: ***p < 0.001. n = 2 slices from 2 mice/group. All data are presented as mean ± SEM. ANOVA, analysis of variance; GSEA, gene set enrichment analysis; IPA, ingenuity pathway analysis; ND, normal diet; WD, western diet; WT, wild-type