Fig. 1.

Symptoms on cotyledons and morphology in vitro of Leptosphaeria maculans “brassicae” (Lmb) or/and Leptosphaeria biglobosa “brassicae” (Lbb). a Examples of symptoms caused by current French field isolates of Lmb and Lbb on cotyledons of two winter-type genotypes (left, line 15–23-4–1 and right, line 18–22-6–1). Pictures were taken 9 days after inoculation and lower panel pictures are reverse face of the upper ones. b Colony morphology of Lmb, Lbb, and mixes of the two species. The isolates were deposited as droplets of 10⁷ spores.mL⁻¹ pycnidiospore suspension, as 10⁵ spores.mL⁻¹ pycnidiospore suspension or as a mix of 10⁷ spores.mL⁻¹ Lmb pycnidiospore suspension + 10⁵ spores mL⁻¹ Lbb pycnidiospore suspension, and grown for 14 days on agar MMII medium