Fig. 3.

Analysis of Brassica napus gene expression following inoculation by Leptosphaeria maculans or/and Leptosphaeria biglobosa. RNA samples were collected at 2, 4, 7, 9, 11, and 15 days post-inoculation (dpi) following inoculation of Brassica napus cv. Darmor-bzh cotyledons. Single-species inoculations (SSI) were done by inoculating pycnidiospore suspensions of Leptosphaeria maculans “brassicae” (Lmb) or Leptosphaeria biglobosa “brassicae” (Lbb) at 10⁷ pycnidiospores.mL⁻¹. Mixed species inoculation (MSI) were done by mixing an equal volume of Lmb and Lbb spore suspensions at 10⁷ pycnidiospores.mL⁻¹. a Principal component analyses (PCA) were done on normalized gene expression of B. napus (centered Log₂(FPKM + 1)). The color code is as follows: SSI by Lbb (purple), SSI by Lmb (red), MSI (black), and control mock inoculation with water (green). b For each of the six time points, the resulting number of differentially expressed genes (DEGs) (p < 0.05, LogFC >|2|) is shown by the histogram which includes the DEG set detected between control samples and Lmb SSI in red and the DEG set detected between the control samples and the “Lbb SSI + MSI” samples in hatched black and purple bars.