Fig. 5.

Analysis of Leptosphaeria maculans or/and Leptosphaeria biglobosa gene expression following inoculation of Brassica napus. RNA samples of Leptosphaeria biglobosa “brassicae” (Lbb; a) or Leptosphaeria maculans “brassicae” (Lmb; b) were collected at 2, 4, 7, 9, 11, and 15 days post-inoculation (dpi) following inoculation of Brassica napus cv. Darmor-bzh cotyledons. Single-species inoculations (SSI) were done by inoculating pycnidiospore suspensions of Lmb or Lbb at 10⁷ pycnidiospores.mL⁻¹ and mixed species inoculations (MSI) were done by mixing an equal volume of Lmb and Lbb spore suspensions at 10⁷ pycnidiospores.mL⁻¹. Principal component analyses (PCA) were done on normalized fungal gene expression (centered Log₂(FPKM + 1)) of Lbb (a) and Lmb (b) either in SSI or in MSI. In a, samples from Lbb SSI are in purple and samples from MSI are in black. In b, samples from Lmb SSI are in red and samples from MSI are in black. Statistical comparisons were done between SSI and MSI samples at each timepoint and for each fungal species. c Histogram of the number of differentially expressed genes (DEGs) detected in Lmb following the comparison between SSI and MSI samples at each sampled date (p < 0.05, LogFC >|2|). According to the statistical tests, no DEGs were found in Lbb when comparing gene expression following SSI and MSI