Fig. 3

High pro-metastatic PC3 and low pro-metastatic LNCaP cell lines cis/trans recruitment by their own EVs embedded in agarose spots: whole spot image analysis (48 h). A Quantification of PC3 migration. B Quantification of LNCaP migration. The percentage of spot area occupied by cells was measured at final time-point images (48 h). C Representative mosaic pictures (× 20) of the PC3 and LNCaP recruitment of each condition. Data from two independent experiments with 3 replicates/condition. Scale bar is 1 mm. The statistical differences are indicated for *p < 0.05, **p < 0.01, and ***p < 0.001, by a Kruskal–Wallis test with a Dunn’s post hoc analysis