Table 1 Comparison of the agarose spot migration assay with other commonly used migration assays
Agarose spot migration assay | Trans-well migration assay | Wound healing assay or similar | Capillary chamber assay | |
|---|---|---|---|---|
Chemotaxis | Yes | Yes | No | Yes |
Stable gradient of the stimuli | Yes | No | No | No |
Measurements | Number of cells Migration area and distance Velocity directionality | Number of cells | Migration area | Number of cells Migration area |
Live imaging | Yes | No | Yes | Yes |
Type of analysis | Endpoint and kinetic | Endpoint | Endpoint and kinetic | Endpoint and kinetic |
Multiple stimuli/well | Yes | No | No | Limited |