Fig. 2

The BAF complex regulates neoblasts and is required for differentiation. A Representative maximum projections of FISH for piwi-1 during RNAi at F4D3. piwi-1+ cells were analyzed according to the time course to left of images. Quantifications of number of piwi-1+ cells in boxed region indicated on diagram below image are shown in graph below the image (n >18). Scale bars, 200μm. B Representative maximum projections of FISH for epidermal progenitor marker prog-2 during RNAi at F4D3. prog-2+ cells were analyzed according to the time course in (A). Quantification of the number of prog-2+ cells in boxed region indicated on diagram below image is shown in the graph below the image (n >18). Scale bars, 200μm. C Representative whole-animal immunostaining for H3P during RNAi at F4D3. Total H3P⁺ cells per worm area were counted at timepoints indicated in (A) and are quantified in the graph below images (n >18). Scale bars, 750µm. D Immunostaining of BrdU with piwi-1 and mat dFISH for planarians subjected to RNAi followed by a BrdU pulse and chase period. An experimental timeline is indicated above images. Stained worms were cross-sectioned post-pharyngeally. Quantifications of the number of piwi-1+/BrdU+ to total piwi-1+ are shown in graph to the right. Scale bars, 100µm. mat is displayed for orientation to the gut. dp is “days post”. E Representative maximum projection of FISH for piwi-1 in BAF RNAi intact heads. piwi-1+ cells anterior to the photoreceptors (location indicated by dashed circles) are quantified in the graph to the right. Scale bars, 100µm. Quantifications are mean \(\pm\) 1 S.D Significance levels in plots: *p<0.05, **p<0.01, ***p<0.001, each n is a representative region from 1 planarian