Fig. 3

RNA and ATAC sequencing indicate the BAF complex is primarily a transcriptional activator. A Schematic of experimental process describing dissociation of worms and purification of X1s (neoblasts), processing for RNA or ATAC sequencing, and general downstream analyses. B Venn diagram demonstrating overlap of differentially expressed transcripts between brg1(RNAi) versus control(RNAi) and smarcc2(RNAi) versus control(RNAi) X1s (adj. p <0.05, no fold cut-off). C Volcano plot displaying commonly dysregulated transcripts between brg1(RNAi) versus control(RNAi) and smarcc2(RNAi) versus control(RNAi) X1s. Average log2 fold changes and adj. p values were used in plot. D Relative expression of neoblast-associated transcripts in brg1, smarcc2, or control RNAi in X1s. * adj. p < 0.05; **adj. p < 0.01. E Venn diagram demonstrating overlap of DA chromatin peaks between brg1(RNAi) versus control(RNAi) and smarcc2(RNAi) versus control(RNAi) X1s (FDR <0.05, no fold cut-off). F Volcano plot displaying commonly differentially accessible peaks shared between brg1(RNAi) versus control(RNAi) and smarcc2(RNAi) versus control(RNAi) X1s. Average fold changes and FDR values were used in plot. G Locations of differentially accessible peaks in X1s during brg1 and smarcc2 RNAi compared to control RNAi with respect to the nearest predicted gene. H ATACseq peaks identified in X1s during RNAi at piwi-1 and bruli loci. The sequence coverage tracks show replicate-averaged, sequence depth-normalized (counts per million, CPM) read coverage for each RNAi condition