Fig. 4

Gut and pharyngeal lineage TFs are accessible and expressed in brg1 and smarcc2 RNAi. A Heatmap of differentially expressed genes in X1s during RNAi. Displayed genes were selected based on their determined or postulated relevance to the lineage indicated on the label on the left. Replicates are displayed independently. B ATACseq peaks identified during RNAi in X1s at the hnf4, nkx2.2, gata4/5/6 and foxA loci, associated with γ-neoblasts and pharynx progenitors. The sequence coverage tracks show replicate-averaged, sequence depth-normalized (CPM) read coverage for each RNAi condition. C dFISH of piwi-1 and hnf4 to mark γ-neoblasts in RNAi conditions at F4D3. Planarians were imaged at the boxed region indicated in the diagram to the left. hnf4+piwi-1+ cells to total piwi-1+ cells are quantified in graph to the right. Scale bar is 50µm. D Immunostaining of BrdU with mat FISH for planarians subjected to experimental course indicated in the timeline above images. Planarians were imaged at the boxed region indicated in the diagram to the left. Quantifications of the number BrdU cells within the gut area (defined by mat expression) through five confocal slices are shown in graphs to the right. Scale bars, 100µm. E dFISH of piwi-1 and foxA in RNAi conditions at F4D3. Planarians were imaged at the boxed region indicated in the diagram to the left. foxA+piwi-1+ to total piwi-1+ cells are quantified in graph to the right. Scale bar is 50µm. Quantifications are mean \(\pm\) 1 S.D Significance levels in plots: *p<0.05, **p<0.01, ***p<0.001, each “n” is a representative region from 1 planarian