Fig. 5

The BAF complex controls access to mesodermal fates and is required for pole regeneration. A ATACseq peaks during RNAi in X1s at PCGs (zic1, prep, ndk, and zic2) and muscle (myoD and foxF1) loci of mesodermal lineages. The sequence coverage tracks show replicate-averaged, sequence depth-normalized (CPM) read coverage for each RNAi condition. B Heatmap of differentially expressed PCGs from RNAi conditions in X1s. Replicates are displayed independently. C Heatmap of differentially expressed muscle-associated genes from RNAi conditions in X1s. Replicates are displayed independently. D Experimental timeline for regeneration experiments in (E and F). The planarian cartoon indicates the location of the amputation sites. E WISH of 3dpa tail and trunk fragments are shown, with the number of worms displaying that phenotype indicated, according to timeline in (D). Scale bars, 200µm. F Representative images of WISH of 10dpa tail and trunk fragments are shown, with the number of worms displaying that phenotype indicated, according to timeline in (D). Scale bars, 200µm. dpa is days post amputation