Fig. 1

Identification of nuclear DELs during sow follicular atresia. a Heatmap of DELs in follicles during sow follicular atresia. RNA-seq data of sow follicles (3–5 mm diameter) were obtained from our previous study [24], and the screening of DELs add a new criterion, that is, reads ≥ 50 in healthy follicles or early atretic follicles. HF, healthy follicle. AF, early atretic follicle. b Prediction of subcellular localization of DELs. Expression scores of DELs in different compartments including cytoplasm, nucleus, ribosome, cytosol, and exosome were predicted using an online tool lncLocator (http://www.csbio.sjtu.edu.cn/bioinf/lncLocator/), and Heatmap was made by Graphpad prism v8.0 software. c Subcellular localization of DELs predicted to be more abundant in the nucleus in sow GCs. Levels of DELs in nuclear and cytoplasm fractions isolated from sow GCs were detected by qPCR. GAPDH and U6 were used for marker genes in the cytoplasm and nucleus, respectively. n = 3. Values are means ± SEM