Fig. 2

Eip63E deficiency leads to early embryonic developmental defects. Ventral views (unless otherwise specified) of whole-mount embryos are shown head to the left. Stage 5–8 embryos are from Eip63E⁸¹ line of unknown genotype. Panels for stages 8–9 onward show Eip63E⁸¹ homozygous mutant embryos. A Staining for single-minded at early embryonic stages (st 5–10) shows no gross difference in midline formation but cellular morphological differences are noticeable. B Staining for engrailed/ invected (4D9 mAb). 18% (n = 48) of total population of stage 8–9 embryos show acute segmentation defects. At stages 10–11, cell bodies appear disorganized (arrowheads) to the extent of loss of hemi-segments division at the midline (wavy arrow) compared to control (straight arrow). C Staining for the HRP antigen. At late stage 11, precocious axon outgrowth (arrows) seen in 36% of deficient embryos (n = 52). Scale bar 50 μm