Fig. 5
From: PFTK1 kinase regulates axogenesis during development via RhoA activation
Eip63E functionally interacts with Rho1 to regulate axogenesis. Ventral views of stage 14 whole-mount embryos stained for BP102 antigen are shown head to the left. Panels show representative images for each specified genotype, A–C representative images of A control embryos, B single heterozygous Eip63E81, and C embryo from Rho172F line showing defective VNC phenotype (D) GFP − embryo from a cross between Eip63E81-deficient line and dominant-negative Rho1 (Rho172F). B, C Images of presumed genotype (see text for details). D Axon defects of intermediate severity in 22.7% of embryos heterozygous for Rho172F and Eip63E81 (GFP −). E–H UAS-elavGal4 approach to drive Eip63E downregulation and constitutive active Rho1 overexpression in neurons in two independent Dmel lines. Lines were then crossed to generate the indicated genotypes. Representative images showing VNC defects from Eip63E downregulated (F) or Rho1.V14 expressing (G) embryos are presented. 86.96% of all embryos from a cross to generate both Rho1.V14 expression and Eip63E81 knockdown (H) show VNC defects. F, G, and H are representative images of presumed genotypes (marked in red, see text for details). Penetrance values from stages 13–17, n as follows: B > 100, C 86, D 22, F 89, G 126, H 69. n represents the total of screened embryos from several different pairings/collection pools. Scale bar 50 μm