Fig. 4

Variability of the analysis by IOCBIO Gel and ImageJ Gel plugin. Variability was estimated as the standard deviation between analysis results performed by different users. A Using the image from Fig. 3A, the variability was found for each gel lane when users were either using IOCBIO Gel or ImageJ Gels plugin. B Summary of variability estimates for all the gel membrane images that were used to estimate either GAPDH, LTCC, or protein content via Ponceau staining. The variability was found to be not statistically significantly different for the two software solutions (paired t-test results shown in the plot, number of compared pairs was n = 117 for each case)