Fig. 1
From: SRSF2 is required for mRNA splicing during spermatogenesis
SRSF2 is essential for male fertility. A Representative images of localization of SRSF2 (green) and MVH (red) in the control and Srsf2cKO testes of 8-week-old mice. The DNA was stained with DAPI. Scale bar: (top) 50 μm; (bottom) 20 μm. B Schematic diagram of deletion of Srsf2 exons 1 and 2 and generation of Srsf2 Δ allele by Stra8 Cre-mediated recombination in male germ cells. C Genotyping PCRs were performed using Srsf2 flox and Srsf2 delta primers. D Quantitative RT-PCR analyses showing Srsf2 mRNA level was decreased. β-actin was used as the internal control. E Western blotting analysis of SRSF2 protein in Srsf2WT and Srsf2cKO total testes of 8-week-old mice. β-actin was detected as an internal control. F Pregnancy rates (%) of plugged wild-type females after mating with Srsf2WT and Srsf2cKO 8-week-old males. G Average litter size of plugged wild-type females after mating with Srsf2WT and Srsf2.cKO 8-week-old males. For this part, 3 mice (8-week-old) of each genotype were used for the analysis. Data are presented as the mean ± SEM. P < 0.05(*), 0.01(**) or 0.001(***)