Fig. 3

Srsf2 deficient germ cells fail to progress into meiosis. A PNA-lectin histochemistry (green), SOX9 (a marker of Sertoli cells, white) and MVH (a marker of germ cells, red) immunofluorescence analysis of the Srsf2^WT and Srsf2^cKO 8-week-old male mice. Scale bar: (top) 50 μm; (bottom) 20 μm. B γH2AX (green) and SYCP3 (red) immunofluorescence analysis of the Srsf2^WT and Srsf2^cKO 8-week-old male mice. Scale bar: (top) 50 μm; (bottom) 20 μm. C PLZF (green) and MVH (red) immunofluorescence analysis of the Srsf2^WT and Srsf2^cKO male mice at P6, P8, P10 and P12. Scale bar, 20 μm. In this part, 3 mice of each genotype were used for the analysis