Fig. 4

Transcriptome and splicing of transcripts changes in SRSF2-null testes. A RNA-seq results showing the reduction of Srsf2 RNA in Srsf2^cKO mice testes. Three independent RNA-seq experiments are shown. B Srsf2^cKO groups rather than to Srsf2^WT groups are clustered together by PCA. C Volcano plot showing transcriptome changes between Srsf2^WT and Srsf2^cKO testes. D Heatmap showing hierarchical clustering of differential expression genes of Srsf2^WT and Srsf2^cKO male mice testes. E GO term enrichment analysis of upregulated genes and downregulated genes. F The five different types of alternative splicing (AS) events. The numbers of abnormal AS events were counted between Srsf2^WT and Srsf2^cKO testes by rMATS software. In this part, 3 mice of each genotype were used for the analysis