Fig. 5

Global landscape of SRSF2-binding sites in mouse testes as revealed by using LACE-seq. A Flowchart of the LACE-seq method. RBP, represents RNA-binding protein. A circled B represents biotin modification. N, represents random nucleotide; V represents A, G or C. IVT, represents in vitro transcription. B Spearman correlation plot between SRSF2 LACE-seq replicates in total testes for assessing the reproducibility of the data. Spearman correlation for the reads counts of each sample was calculated from two replicates. C Genomic distribution of SRSF2 binding sites in testes. CDS, coding sequence. UTR3, 3′ untranslated region. UTR5, 5′ untranslated region. D Schematic analysis showing the distribution of SRSF2-binding sites in the vicinity of the 5′ exon–intron and the 3′ intron–exon boundaries (500 nt upstream and 500 nt downstream of 3′SS; 500 nt upstream and 500 nt downstream of 5′SS). E SRSF2-binding motifs identified by LACE-seq in mouse testes. F GO enrichment map of SRSF2-binding genes. G Network analysis of the enriched GO terms of SRSF2-specific targets