Fig. 6

The expressions of key SRSF2-binding genes involved in the spermatogenesis change after Srsf2 KO. A Correlation analysis between the RNA-seq and LACE-seq. GO analysis of the significantly upregulated genes and SRSF2-binding genes. B Network analysis of the enriched GO terms of the significantly upregulated genes and SRSF2-specific targets. C Correlation analysis between the RNA-seq and LACE-seq. GO analysis of the significantly downregulated genes and SRSF2-binding genes. D Network analysis of the enriched GO terms of the significantly downregulated genes and SRSF2-specific targets. E Quantitative RT-PCR validation of the expression of genes involved in (B). β-actin was used as the internal control. Data are presented as the mean ± SEM. P < 0.05(*), 0.01(**) or 0.001(***). F Quantitative RT-PCR validation of the expression of genes involved in (D). β-actin was used as the internal control. Data are presented as the mean ± SEM. P < 0.05(*), 0.01(**) or 0.001(***)