Fig. 6

Screenshots of interactive graphical outputs from MicrobIEM's basic microbiome analysis options. The interactive graphical user interface of MicrobIEM facilitates basic microbiome analysis. Implemented are alpha diversity analysis (A), beta diversity analysis (B), and analysis of the taxonomic composition (C) based on metadata and an easy and dynamic sample selection within the tool. As an example, differences in microbiome alpha diversity at week 0 and week 8 (A) and in global microbiome structure (B) are shown by lesional (LS) versus non-lesional (NL) skin, while C displays the sample composition per patient on genus level at a selected timepoint (week 8). Dots in A and B indicate individual samples. Boxes in A denote the median and interquartile range (IQR, distance between 25 and 75th percentile), and whiskers represent values up to 1.5 times the IQR. Ellipses in B denote 95% confidence intervals around cluster centroids based on a multivariate t-distribution. Bars in C show the microbiome composition of the ten most abundant genera at one timepoint per patient, while remaining genera are summarized as “Others”