Fig. 2

Experimental design and scRNA sequencing preprocessing (EDSSP) revealed that DRG neurons have innate subtypes. A Schematic diagram of the fluorescein labeling process and representative image of acute-dissociated DRG neurons labeled with DiO (green) and DiI (red). Scale bar, 100 μm. B Schematic diagram of the procedure used to rapidly dissociate and isolate single-labeled DRG neurons for single-cell RNA-seq profiling. dbi, days before injury; dpi, days post-injury. C, D Unsupervised cell clustering is processed by Seurat and visualized by UMAP. The 10 clusters were annotated based on known/verified cluster markers and sorted into 5 known innate subtypes. E Gene expression dot map of the 10 clusters. DRG, dorsal root ganglion; DRGs, dorsal root ganglion neurons; PEP, peptidergic nociceptor; NF, Nefh⁺ A-fiber low-threshold mechanoreceptor; NP, nonpeptidergic nociceptor; SST, somatostatin⁺ pruriceptors; c_pLTMR, putative low-threshold c-mechanoreceptor; cLTMR, C-fiber low-threshold mechanoreceptors