Fig. 3

EDSSP indicated that Adcyap1 is likely highly associated with injury and repair processing. A The model of the first step of EDSSP. Processed by Seurat, the DRG neurons are sorted by known/verified cluster markers into several clusters and then sorted by time-design and surgical-design into several sub-modules. B The ratio changes of sub-types DRG neurons (DRGs) show that PEP1 may relate with injury and repair processing. C The model of the second step of EDSSP. Processed by WGCNA, the crushed DRG neurons of PEP1 are sorted into 4 time points according to the experimental design. D Module-trait relationships reveal that there are 4 modules of genes changed by time-design. E Venngram of Pain genes and 3 PEP subtypes’ high-expression genes. F The metascape results of Pain module genes. G The expression of Adcyap1 (left) and CD9 (right) in the PEP1 DRGs for the sham (n = 8) and SNC-1 d (n = 10 for Adcyap1; n = 9 for CD9) groups by single-cell qPCR, unpaired t test. *P < 0.05. n.s., no significant difference. H RNAscope® images of rat DRGs stained with probes and antibody (left), Adcyap1 (green), Map2 (red), and DAPI (blue). Representative sections from sham and SNC 1-day, 3-day, and 7-day DRGs are shown. RNAscope statistical graph of Adcyap1 in different time points after SNC (Right)