Fig. 5

Examples of structures of novel intragenic transcripts. Nanopore sequencing results of products of 5′/3′ RACE or RT-PCR for InSETe-24 and InSETe-110 inside correspondingly MAML2 (a) and ESYT2 (b) genes. The bottom portions represent zoom-in views of the boxed regions above. For ESYT2, only representative transcripts are shown: for full depiction of 5′/3′ RACE results see Additional file 1: Fig. S5. Known genes annotations are in blue and based on the GENCODE Genes track from the UCSC Genome Browser. The InSETes are in yellow and marked by the red arrows. Structures of novel transcript InSETT-2 or the InSETT-8 cluster of novel transcripts (sharing InSETe-110) found in correspondingly MAML2 and ESYT2 loci by 5′/3′ RACE (red) and RT-PCR (black) are shown. IACFs and CACFs are in brown and represent unique insertions and merged clusters from only the drug survival system. Black arrows indicate the RACE or RT-PCR primers