Fig. 2

SmuCas9 PAM-interacting domain chimeras or evolved Nme2Cas9 variants expand the targeting scope of Nme2ABE8e-797. a Schematic diagram of Nme2ABE8e-797, Nme2ABE8e^−Smu and Nme2ABE8e^−C. b Comparison of A-to-G editing efficiency produced by Nme2ABE8e-797, Nme2ABE8e-Smu and Nme2ABE8e-C using Sanger sequencing at 8 PAM-matched N4CN sites in HEK293T cells. Bars represent mean values, and error bars represent the s.d. values of three independent biological replicates, with *, ** and *** representing P < 0.05, 0.01 and 0.001, respectively (two-tailed unpaired t test). c Data from (b) were aggregated and replotted. Comparison of average A-to-G editing efficiency produced by Nme2ABE8e^−Smu and Nme2ABE8e^−C in HEK293T cells. Bars represent mean values, and error bars represent the s.d. of three independent biological replicates