Fig. 5

RNA editing landscape in X. tropicalis. A Venn diagram showing the number of A-to-I editing sites found in three different studies, which are indicated by the initials of the last authors. JBL refers to the datasets reported by Jin Billy Li [14], KW refers to the datasets reported by Karl Wotton [75], and MKK refers to the datasets reported by Mustafa K. Khokha [76]. B ADAR motif in X. tropicalis based on our curated list of editing sites. C Genomic locations of editing sites in X. tropicalis. Expectedly, most editing events were found in non-coding regions of the genome. An appreciable percentage also lie within intergenic regions. D Editing in repetitive regions of the X. tropicalis genome. Like X. laevis, minority of the ADAR targets in X. tropicalis were found in repeats. The pie chart shows the distribution of A-to-I editing sites in various repeat families. Twenty-six annotated repeat families contained comparatively few editing events and thus were grouped together in a single slice of the pie chart. E PCA plot based on gene expression values from the JBL study showing segregation of embryonic samples according to developmental stages. F Transcript levels of ADAR enzymes across development in the JBL study. The ADAR expression values are provided in Additional File 2. G Global editing index measured across all repeat families in the JBL study. H Editing index for each individual repeat family in the JBL study. While ADAR activity was variable across the repeat families, it was consistently higher during the beginning stages of development regardless of repeat type. Twenty-six annotated repeat families contained comparatively few editing events and thus were grouped together for calculation of the index. I Hierarchical clustering of editing levels. Each row is a different editing site, while each column is a different developmental stage interrogated in the JBL study. J Many transcriptomic loci were targeted by ADARs in only a single developmental process. Top: Heatmap depicting the editing rates of these process-specific sites. Bottom: Genomic locations of the process-specific sites