Fig. 1
From: The meaning of ubiquitylation of the DSL ligand Delta for the development of Drosophila
Generation of the DlattP alleles. A Generation and use of DlattP. Exon 6 of Dl was replaced by an attP landing site. This replacement creates a Dl null mutant allele DlattP. The attP site allows the insertion of modified exon6-variants. B, B’ Cuticle preparations of wt (B) and DlattP (B’) embryos. The DlattP mutant flies possess only a small patch of dorsal cuticle (arrowhead in B’). This neurogenic phenotype is characteristic for mutants of genes encoding Notch pathway components. C–D’’ Expression of DlattP-Dl-HA (C–C’’) and DlattP-DlK2R-HA (D–D’’) in comparison to Dl::GFP. E, E’ The wing region of a wing disc bearing Dl-HA homozygous clones (bright green, Dl-HA). The clone is outlined in white and yellow. The clone is next to a clone homozygous for endogenous Dl (Dl, no green). E’ The expression levels of both Dl-variants is indistinguishable, indicating that the knock-in of a HA-modified exon 6 does not affect the levels of expression