Fig. 4
From: The meaning of ubiquitylation of the DSL ligand Delta for the development of Drosophila
The Ks in its ICD are required for the full activity of Dl. A, B Gbe + Su(H) expression in DlattP-Dl-HA and DlattP-DlK2R-HA/Df discs. Four stripes are recognisable in the wt disc in A. The arrow points to stripe3 which is decreased in DlattP-DlK2R-HA/Df discs. C–D’’ A wing disc bearing homozygous DlattP-Dl-HA (bright green outlined in white) andDlattP-DlK2R-HA (no green) twin clones.C Overview. D–D’’’’ Magnification of the notal area highlighted in C with the arrowhead. The homozygous Dl-HA clone is outlined in white or yellow. Although DlattP-Dl-HA accumulates to lower levels than DlattP-K2R-HA in the plasma membrane (D’), Gbe + Su(H) is stronger expressed in DlattP-Dl-HA homozygous territory (D’’), indicating that DlattP-Dl-HA can activate the Notch pathway more strongly and that also stripe2 is affected by the loss of the Ks in the ICD of Dl. D’’’, D’’’’ pixel density measurement reveals the Gbe + Su(H) is increased in the clone homozygous for Dl-HA, whereas the HA-signal drops. The converse is true in the adjacent DlK2R-HA homozygous clone. s: start of the measurement. See also Additional file 1: Fig. S3 for more examples. E–E’’’ Measurement in a control wing disc with the same genotype as in C–D'’’’ without clones for comparison. F, G’ Expression of Wg in DlattP-Dl-HA/DfandDlattP-DlK2R-HA/Df discs. The arrow points to the expression of Wg along the D/V-boundary. H–L induction of ectopic Wg expression by expression of Fng with ptcGal4. The length of the ectopic stripe of Wg expression is strongly reduced in DlattP-DlK2R-HA/Df compared to DlattP-Dl-HA/Df discs (arrow). L Quantification of the length of the ectopic stripe of Wg expression induced by Fng in the DlattP variants. The length is measured by the number of cells in the ectopic Wg stripe (rectangle, n = 12 for Dl-HA and n = 18 for DlK2R)