Fig. 7
From: The meaning of ubiquitylation of the DSL ligand Delta for the development of Drosophila
A–D’ Ubi-independent Dl-signalling at the stripe3 of Gbe + Su(H) expression domain. A Expression of Gbe + Su(H) in a wildtype wing disc. The arrowhead points to stripe3. B Residual expression of stripe3 is observed in mib1 mutant wing discs (arrowhead). C–D’ Stripe3 is also present in mib1-mutant DlattP-Dl-HA/Df (C, C’) and mib1-mutant DlattP-DlK2R-HA/Df (D, D’) discs (arrowhead). E,E’ A disc bearing a large lqf-mutant clone covering the region of stripe3 expression (arrowhead). The clone is labelled by absence of GFP. The expression of stripe3 is abolished. F, G The wing phenotype of DlattP-Dl-HA/Dfandmib1DlattP-DlK2R-HA/Df flies upon reduction of lqf activity. The wing of mib1DlattP-DlK2R-HA/Dflqf flies is much more severe than that of DlK2R-HA/Df flies (compare with Fig. 2G).H, I Suppression of the phenotype of homozygous DlattP-DlK2R-HA flies by elevation of the copy number of lqf. H The original number of tarsal segments is re-established if two additional copies are present. The arrow points to the incomplete formation of the joint between tarsal segments 3 and 4. Note, the homozygous flies normally had only three discernible segments due to the fusion of segments 3 + 4 and 1 + 2 (see Fig. 2K). I The wing phenotype of homozygous DlattP-DlK2R-HA is strongly suppressed by elevation of the levels of Lqf (compare with Fig. 2I)