Fig. 10

Interactions of T. vaginalis Mdm12 with phospholipids. A Example of docking of phosphatidylinositol 4-phosphate (PBD ID T7M) into TvMdm12 as predicted by AutoDock Vina v1.2.3 [36]. Green, red, orange, and white represent carbon, oxygen, phosphate, and hydrogen, respectively. B Boxplot of ligand binding affinities. Individual colored boxes illustrate groups of phospholipids. The single box represents Inter quartal range (IQR) with a horizontal line showing the median. Whiskers represent maximum (Q3 + 1.5*IQR) and minimum (Q1 − 1.5*IQR) values. Dots indicate outliers. The significance of the difference among the groups was assessed by ANOVA (F-value = 4.56, P-value = 0.0003, Degree of freedom = 6) and followed by a post hoc test (Tukey). Stars indicate the significance of the group versus PIP (p-value< 0.05 = *, p-value< 0.01 = **, p-value 0.001 = ***). CL, cardiolipin; PA, phosphatidic acid; PC, phosphatidylcholine; PE, phosphatidylethanolamine; LA, lysophosphatidic acid; PG, phosphatidylglycerol; PtdInsP, phosphatidylinositolphosphate. C Binding of recombinant T. vaginalis TvMdm12 to lipids by protein-lipid overlay assay. Membrane lipid strips were incubated with recombinant TvMdm12. The binding of TvMdm12 to immobilized lipids in the spots was detected by mouse monoclonal α-His antibody and α-mouse antibody conjugated with horseradish peroxidase. TG, triglyceride; DAG, diacylglycerol; PA, phosphatidic acid; PS, phosphatidylserine; PE, phosphatidylethanolamine; PC, phosphatidylcholine; PG, phosphatidylglycerol; CL, cardiolipin; PtdIns, phosphatidylinositol; PtdIns(4)P, phosphatidylinositol 4-phosphate; PtdIns(4,5)P2, phosphatidylinositol 4,5-bisphosphate; PtdIns(3,4,5)P3, phosphatidylinositol 3,4,5-trisphosphate; SGC, 3-sulfogalactosylceramide