Fig. 2
Distribution of ERMES subunits and key enzymes of mitochondrial phospholipid metabolism in anaerobic protists with examples of aerobic relatives. Green and empty squares indicate the presence or absence of the gene. Numbers indicate the number of paralogs. Green names highlight free-living species. Classification is based on homology searches, cell localization predictions, and phylogenetic analysis (Additional file 3: Table S2, Fig. 9, Additional file 2: Fig. S3 and S4). The question mark indicates a protein with inconclusive classification. P2, Porin2; Nvj2, nucleus–vacuole junction 2 protein; Psd1, phosphatidylethanolamine decarboxylase; CLST, cardiolipin synthase with transferase mechanism [29]