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Fig. 3 | BMC Biology

Fig. 3

From: The divergent ER-mitochondria encounter structures (ERMES) are conserved in parabasalids but lost in several anaerobic lineages with hydrogenosomes

Fig. 3

Cellular localization of T. vaginalis TvMmm1 observed by confocal microscopy. Hemagglutinin (HA)-tagged recombinant proteins, TvMmm1a, TvMmm1b, and disulfide isomerase (PDI, ER marker) were visualized using mouse α-HA antibody (green). The malic enzyme (ME), a hydrogenosomal marker, was visualized with rabbit α-malic enzyme (red) antibody. Alternatively, HA-tagged recombinant TvMmm1a or TvMmmb were co-expressed with recombinant V5-tagged PDI and visualized using mouse α-HA (green) and rabbit α-V5 antibodies (red). The nucleus was stained with DAPI (blue). Bar = 5 μm

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BMC Biology

ISSN: 1741-7007

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