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Fig. 4 | BMC Biology

Fig. 4

From: The divergent ER-mitochondria encounter structures (ERMES) are conserved in parabasalids but lost in several anaerobic lineages with hydrogenosomes

Fig. 4

Cellular localization of T. vaginalis ERMES components observed by confocal microscopy. A Colocalization of TvMmm2a, TvMmm2b, and TvMdm12 with hydrogenosomal malic enzyme (ME). Hemagglutinin (HA)-tagged TvMmm2a, TvMmm2b, and TvMdm12 were visualized using mouse α-HA antibody (green). The malic enzyme (ME) was visualized with rabbit α-malic enzyme (red) antibody. B Interactions of TvMmm2b with TvMdm12 and TvMmm1a. HA-tagged TvMdm12 or TvMmm1a were co-expressed with recombinant V5-tagged TvMmm2b and visualized using mouse α-HA antibody (green) and rabbit α-V5 antibody (red). The nucleus was stained with DAPI (blue). Bar = 5 μm

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