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Fig. 7 | BMC Biology

Fig. 7

From: The divergent ER-mitochondria encounter structures (ERMES) are conserved in parabasalids but lost in several anaerobic lineages with hydrogenosomes

Fig. 7

Cellular localization of T. vaginalis Porin2. A Immunofluorescent microscopy. Hemagglutinin (HA)-tagged recombinant Porin2 and hydrogenosomal marker malic enzyme (ME) were visualized using mouse α-HA (green) and rabbit α-malic enzyme (red) antibodies, respectively. The nucleus was stained with DAPI (blue). B Western blot analysis of cellular fractions isolated from the lysate of HA-tagged Porin2 expressing T. vaginalis cells. Cellular fractions were separated by differential and Percoll gradient centrifugation. L, cell lysate; C, cytosolic fraction; LDV, low-density vesicles; H, hydrogenosomal fraction. Hydrogenosomal fraction was treated with proteinase K (HpK) or proteinase K with Triton X-100 (HpKTX). Porin2, OsmC (hydrogenosomal marker), C-tail anchored protein 7 (CTA7), PDI (ER marker), and cytosolic malic enzyme (cyt. ME, cytosolic marker) were detected by mouse α-HA, rat polyclonal α-OsmC, rat polyclonal α-CTA7, rat polyclonal α-PDI, and mouse α-cyt. ME, respectively

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BMC Biology

ISSN: 1741-7007

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