Fig. 9
T. vaginalis possesses phosphatidylglycerol phosphate synthase (PGPST) but not cardiolipin synthase (CLST). A Phylogenetic analysis of PGPST and CLST. The maximum likelihood (ML) tree was constructed using IQ-TREE (Best fit; LG + F + I + G4 model) with 40 sequences and 186 sites. Bootstrap support values and aBayes posterior probability were calculated using 1000 replicates each. The support values are represented in the order of aBayes (posterior probability value)/ML ultra-fast bootstrapping)/ML Non-parametric bootstrapping. B Cellular localization of T. vaginalis PGPST. Hemagglutinin (HA)-tagged PGPST was expressed in T. vaginalis and visualized using mouse α-HA antibody (green). The malic enzyme (ME), a hydrogenosomal marker, was visualized with rabbit α-ME enzyme (red) antibody. Alternatively, HA-tagged PGPST was co-expressed with V5-tagged protein disulfide isomerase (PDI), an ER marker that was detected with rabbit α-V5 (red) antibody. The nucleus was stained with DAPI (blue). DIC, differential interference contrast. Bar = 5 μm