Fig. 1

Timescale conversion of Pre-B cells into Macrophages. a Immunofluorescence staining of C/EBPα showing cellular localization of C/EBPα proteins at different time points. b Western blot analysis showing C/EBPα protein level in nuclear and whole cell extracts upon β-estradiol induction. c FACS analysis of the percentage of cells that express Pre-B cell-specific surface marker, CD19, and macrophage-specific cell surface marker, CD11b. d Gene expression level (transcripts per million) of genes encoding histone3. e Gene expression level (transcripts per million) of Cebpa gene. f Fuzzy c-means clusters of differentially expressed genes from 0h, 1h, 12h, and 48h during trans-differentiation. g Gene expression level (transcripts per million) of Pre-B cell-specific genes (Rag1, Rag2, Hdac7, Pax5) (blue), and macrophage-specific genes (Cebpb, Csf1r, Fcgr1, Itgam) (red) during Pre-B trans-differentiation