Fig. 1

Schematic representation of the experimental design. Six different mutation accumulation lines were constructed. Two lines were maintained in the control condition (blue), two lines were exposed to cisplatin at a low dose of 75 mM (green) and two lines were exposed to a higher dose of 150 mM of cisplatin (red). Each line consisted of a pre-epimutation accumulation generation (F0) that was submitted to 20 successive bottlenecking events for which 2 worms were randomly picked from the previous generation and used as founders of the next generation. Embryos were sampled every two generations for molecular analysis. Figure realised using BioRender and Affinity Designer 2