Cisplatin exposure alters tRNA-derived small RNAs but does not affect epimutations in C. elegans

Table 2 Stepwise analysis of the association of gene expression changes and tRNA epimutations in the different exposure conditions

Condition	Test	Association tested	Background	Odds ratio	p-value
Control	1	Gene exp. change and tRNA epimutations	All genes	Infinite	9.58e − 04
Control	2	Inherited gene exp. change and tRNA epimutations	All genes with RNA-seq changes	0.34	0.03
Control	3	Inherited gene exp. change and simultaneous tRNA epimutations	All genes with RNA-seq changes	0	1
Control	4	Inherited gene exp. change and simultaneous tRNA epimutations	All genes with RNA-seq changes and tRNA epimutations	0	1
Low dose	1	Gene exp. change and tRNA epimutations	All genes	Infinite	8.86e − 05
Low dose	2	Inherited gene exp. change and tRNA epimutations	All genes with RNA-seq changes	0.65	1.00e + 00
Low dose	3	Inherited gene exp. change and simultaneous tRNA epimutations	All genes with RNA-seq changes	0.23	7.30e − 03
Low dose	4	Inherited gene exp. change and simultaneous tRNA epimutations	All genes with RNA-seq changes and tRNA epimutations	Infinite	1.00e + 00
High dose	1	Gene exp. change and tRNA epimutations	All genes	19.78	2.81e − 04
High dose	2	Inherited gene exp. change and tRNA epimutations	All genes with RNA-seq changes	1	1
High dose	3	Inherited gene exp. change and simultaneous tRNA epimutations	All genes with RNA-seq changes	1.61	0.70
High dose	4	Inherited gene exp. change and simultaneous tRNA epimutations	All genes with RNA-seq changes and tRNA epimutations	Infinite	1

Cells with p-value < 0.05 indicate significantly increased odds of association, and cells with p-value > 0.05 in grey indicate no significant association. Odds ratios and p-values were calculated with Fisher’s exact test. Data from the two lineages for each condition were combined (N = 2)

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