Fig. 2

Genome-wide landscape of ERβ chromatin binding in mouse ovary. A ERβ chromatin-binding sites in mouse ovary per ChIP-seq replicate (n = 3 replicates, each with 14 pooled ovaries from 7 mice) in relation to corresponding input samples, visualized in a heatmap. B Venn diagram of detected ERβ-binding sites in ChIP-seq triplicates normalized against input. Sites present in at least two replicates, colored in blue, were used in further analysis. C The genomic distribution of ERβ-binding sites. All regions, except intergenic regions, were significantly enriched (p < 0.005). D Top-enriched DNA motifs among ERβ-bound genomic sequences, identified using HOMER de novo motif analysis (sorted by p-value). % sequences represent the percentage of ChIP:ed sequences (bound sites) that have a particular motif. The percentages will not add up to 100% as each sequence can have more than one motif. E Enriched biological functions among genes nearest ERβ chromatin-bound sites (within −50 kb to +2 kb)