Fig. 3

Impact of ERβ on the ovarian transcriptional landscape. A Volcano plot of ERβ-regulated genes. Genes were considered differentially expressed when FDR < 0.05 and log₂FC >|0.4| (n = 5 WT, n = 4 ERβKO). B Top-10 enriched biological pathways of the down- (blue) and upregulated (red) DEGs. The size of the bubbles corresponds to the enrichment score (-log₁₀(p-value)). C All significantly enriched biological pathways visualized in semantic space. The size and color of the bubbles correspond to the enrichment score (-log₁₀(p-value)) and gene count, respectively. D Bar chart presenting the projected cell type abundance in the ovary of WT and ERβKO mice (Wilcoxon rank-sum exact test)