Fig. 4

Identification of direct ERβ-regulated genes and biological pathways in mouse ovary. A Venn diagram representing the genes overlapping between ERβ ChIP-seq in WT ovaries (n = 3 replicates, each with 14 pooled ovaries from 7 mice) and RNA-seq performed in WT (n = 5) and ERβKO ovaries (n = 4). B Circle plot representing the top-10 enriched biological functions of the 245 overlapping genes between ChIP-seq and RNA-seq (from A). For each biological function, up- (red) and downregulated (blue) genes are represented. C ChIP peaks show enrichment of ERβ at chromatin by genes involved in specific functions. D ChIP enrichment at binding regions nearest to Esr2 and Nr5a2. All tracks are set to the same Y-axis height for the ChIP-seq and input. E The top-10 enriched biological functions of the regulated genes identified by both ChIP-seq and microarray (before and after ovulatory signal, from [29]). The size of the bubbles corresponds to the enrichment score (-log₁₀(p-value)). F ERβ ChIP signal at Fshr and Tgfbr1